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Biomarker detection using electrochemical method with microfluidic chip
Klepáčová, Ivana ; Svoboda, Ondřej (referee) ; Neužil, Pavel (advisor)
The thesis is focused on the development of the electrochemical system with microfluidic platform for the detection of multiple biomarkers. It analyses the use of biomarkers for the early diagnosis of cancer. The theoretical part contains basic information about voltammetric methods and microfluidic systems. The practical part provides solutions to the microfluidic chips, including the description of the used materials, designs, methodologies of preparation and conclusions from the testing of the manufactured microfluidic systems. The thesis describes the lock-in electrochemical system which measures the response of 4 electrochemical cells simultaneously. For the electrochemical system measurements, an electrochemical chip consisting of 64 electrochemical cells was used. The results of the analysis include the processing of the system tests and detected voltammetric curves of the Fe2+/Fe3+ solution and cysteine.
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Cell transplantation methods in cardiology
Kukhta, Dziyana ; Hežová,, Reneta (referee) ; Skopalík, Josef (advisor)
Tato diplomová práce se zabývá tkáňovým inženýrstvím, zejména tvorbou homogenní, izotropní a planární vrstvy buněk srdečního svalu pomocí dvou technologii:”scaffold-based” a ”scaffold-free”. Nejprve popsaný histologie srdeční stěny, buňky srdečního svalu a buněčné kultury. Následuje popis tkáňového inženýrství, který zahrnuje technologii “cell sheet” a tkáňové inženýrství na bázi scaffoldů. Na konci teoretické části je popsána aplikace tkáňového inženýrství a buněčná vizualizace. Praktická část věnovaná tvorbě planární buněčné vrstvy z kardiomyocytů a fibroblastů s využitím informací z teoretické části.
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Microfluidic models of cell membranes
Haluzová, Kateřina ; Dzik, Petr (referee) ; Pekař, Miloslav (advisor)
This work deals with artificial cellular membrane models, their various types, production and usage. It also summarises basic matherials for production of microfluidic chips. Experimental part is focused on optimalization of production of polydimethylsiloxane chip suitable for free-standing lipid bilayers. A preparation workflow was developed which includes casting on a silicon wafer, removing bubbles from the PDMS, curing it at 80 °C for 1 h, plasma bonding, annealing at 150 °C for 30 min and storage. Using fluorescence microscopy, the prepared chips were found to be strong and sealed.
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Thin metal coating of polymer materials
Zatloukal, Filip ; Furmann, Denis (referee) ; Nečas, David (advisor)
The study of compliant contacts from a tribological standpoint is very complicated due to low reflectivity and electrical conductivity of materials such as polymers or rubber. These properties significantly limit the possibilities of using conventional methods which are being used to study the film formation and lubricant flow. To enable the use of the conventional methods it is necessary to apply a thin reflective layer on the non-reflective surface. This work evaluates the current literature dealing with the coating of polymeric materials. A method was chosen based on the analysis of the currently available technologies, which is used to apply a thin reflective layer on the polydimethylsiloxane sample. This part is followed by a feasibility study to clarify the effects of the coating on friction processes. This work can significantly contribute to the field of compliant contacts, especially in terms of clarifying the mechanisms of lubrication.
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Advanced membrane systems
Gjevik, Alžběta ; Márová, Ivana (referee) ; Mravec, Filip (advisor)
The diploma thesis deals with cellular membrane model preparation on microfluidic devices. It summarizes means of microfluidic device fabrication, phospholipid bilayer formation mechanisms, optimization techniques and characterization methods of those systems. It focuses on free-standing planar lipid bilayers which are easily accessible by a number of different characterization methods and at the same time exhibit good stability and variability. The aim of this work is to design and prepare a microfluidic chip on which a planar lipid bilayer can be prepared. It therefore presents microfluidic device prepared by soft lithography of PDMS adapted for model membrane formation by self-assembly of phospholipids at the interface of aqueous and organic phases created by the architecture of the microfluidic device. Formation of the model membrane was visualized by optical microscopy and fluorescence-lifetime imaging microscopy.
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Membrane modeling in microfludic chip
Konečná, Tereza ; Pekař, Miloslav (referee) ; Venerová, Tereza (advisor)
This work focuses on microfluidic chips suitable for modeling phospholipid membranes, their design, and possible applications. It also provides a more detailed specification of selected techniques for creating membranes on a chip. The experimental part deals with the production of chips from polydimethylsiloxane, the optimization of infusing of solutions into the chip, and the formation of membranes inside microfluidic channels using the selected model phospholipid – asolectin. The entire process of membrane modeling was visualized using optical and fluorescence microscopy. In conclusion, the success of the developed method is evaluated, including the suitability of asolectin for the formation of model membranes, and solutions to existing problems are proposed.
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Measurement of force generated by living cells
Lukášová, Veronika ; Skácel, Josef (referee) ; Fohlerová, Zdenka (advisor)
This thesis deals with methods used to measure cell traction forces. In order to study and measure the forces it is important to understand the mechanotransduction process. This is described in the theoretical part of the thesis along with an explanation of the adhesive capacity of cells. Next are presented the methods used to monitor traction forces: collagen contraction, tissue pillars, traction force microscopy in 2D and 3D, and measurements on micro- and nano-pillars. The practical part of the thesis is focused on determining the magnitude of the traction forces, which can be calculated from the knowledge of the pillar deflection and the spring constant of the material. The practical part includes the processing of the light microscope images and the resulting procedure for calculating the magnitude of the traction forces.
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Microfluidic models of cell membranes
Haluzová, Kateřina ; Dzik, Petr (referee) ; Pekař, Miloslav (advisor)
This work deals with artificial cellular membrane models, their various types, production and usage. It also summarises basic matherials for production of microfluidic chips. Experimental part is focused on optimalization of production of polydimethylsiloxane chip suitable for free-standing lipid bilayers. A preparation workflow was developed which includes casting on a silicon wafer, removing bubbles from the PDMS, curing it at 80 °C for 1 h, plasma bonding, annealing at 150 °C for 30 min and storage. Using fluorescence microscopy, the prepared chips were found to be strong and sealed.
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